Fig. 2.

PTHrP-creER marks DF mesenchymal progenitor cells in vivo. (A–F) Lineage tracing of P3 PTHrP-creER⁺ DF cells. Yellow arrowheads in A indicate tdTomato⁺ DF cells in periapical areas. (B) Cytokeratin 5 (CK5) staining. (C) Yellow dotted line in (1) denotes HERS. (D) EdU was administered shortly before analysis. Arrowheads in D denote EdU⁺tdTomato⁺ DF cells. (E) Blue arrows in (3) indicate Col1-GFP⁺tdTomato⁺ osteoblasts in cryptal bones. (F) Yellow arrowheads in (4) indicate GFP⁺tdTomato⁺ cementoblasts on root surface. Pound signs in (4) denote tdTomato⁺ osteocytes in interseptic cryptal bones. Asterisks in (5) indicate tdTomato⁺ osteocytes in interradicular cryptal bones. C, cementum; D, dentin. PDL, periodontal ligament. [Scale bars: 200 µm (Left), 20 µm (Right).]