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Size-exclusion chromatography of enriched HM. (A) EM polysaccharides were solubilized in 10% NaOH overnight, neutralized, and analyzed by size-exclusion chromatography. Arrows indicate the elution of Dextran standards of known sizes. Data show the Glc and Man content (stacked). (B) An equal amount of the EM material used in A was digested with β-mannanase before SEC analysis.
