Fig. 3.

MO domain of MICAL3 plays critical roles in tumor sphere formation. (A) Schematic of WT MICAL3 and mutant (3G3W and N1) constructs. (B) Measurement of H₂O₂ production using lysates of HEK293T cells transfected with the indicated constructs described in A in the presence of 200 µM NADPH. As a positive control, cells were treated with 1 µM H₂O₂; n = 3. (C and E) Representative phase contrast images of tumor sphere formation by MCF7 or patient-derived BCCs transiently transfected with the indicated constructs cultured in SCM. (Scale bar: 100 µm.) (D and F) Tumor sphere formation by MCF7 or patient-derived BCCs transfected with the indicated constructs cultured in SCM; n = 3. Data are shown as mean ± SD. *P < 0.05, ***P < 0.001 by Student’s t tests.