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. 2018 Dec 24;116(2):490–495. doi: 10.1073/pnas.1714990116

Fig. 1.

Fig. 1.

Whitefly preinfestation improves the susceptibility of tobacco. (A and B) Whiteflies were allowed to feed on whitefly-preinfested and -uninfested control tobacco plants for 3 d. The survival (A) and fecundity (B) of whiteflies were determined to assess host plant susceptibility. Each treatment included six plants, and each plant had two clip cages (n = 12). (C–E) The mean levels of SA (C), JA (D), and jasmonoyl-isoleucine (JA-Ile) (E) in the control and preinfested tobacco plants were measured (n = 6). (F and G) Expression levels of marker genes in SA- (F) and JA- (G) signaling pathways of control and preinfested tobacco plants: phenylalanine ammonia lyase (PAL), nonexpresser of PR genes 1 (NPR1), pathogenesis-related protein 1a (PR1a); x3 fatty acid desaturase 7 (FAD7), coronatine-insensitive 1 (COI1), and plant defensin (PDF1.2) (n = 5 to 6). (H and I) The effect of exogenous SA application on whitefly survival (H) and fecundity (I). Eight plants were used for each treatment, and each plant included two clip cages (n = 16). The data shown are mean ± standard error (SE). The letters above the bars indicate significant differences among different treatments at P < 0.05 [nested analysis of variance (ANOVA) for whitefly bioassays and one-way ANOVA for other experiments].