Fig. 4.

Three-dimensional models of a single cMDH monomer from Echinolittorina malaccana, E. radiata, Lottia austrodigitalis, L. digitalis, Chlorostoma funebralis, and C. montereyi. Red-colored ribbons identify the regions in which the increase in simulation temperature led to a significant change in structural movements [indexed by a change in rms fluctuation (rmsf) over the equilibration state (10–20 ns) greater than 0.5 Å]. Simulation temperatures were 15 °C and 57 °C for the genus Echinolittorina and 15 °C and 42 °C for the other orthologs (2). The locations of the two mobile regions are shown by dashed lines in E. malaccana cMDH monomer. The variable sites between species within each genus are indicated by yellow spheres.