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. Author manuscript; available in PMC: 2019 Jan 12.
Published in final edited form as: FEMS Microbiol Lett. 2009 Apr 27;295(2):238–244. doi: 10.1111/j.1574-6968.2009.01605.x

Fig. 3. Sensitivity of P. aeruginosa ahpCF versus katB mutants to H2O2 in biofilms.

Fig. 3.

The same conditions used in Fig. 2 were used to grown ahpC and katB mutant biofilms. Confocal laser scanning microscopic images of top and saggital views of mature 3-day old biofilms were obtained in (A) untreated bacteria and (B) after treatment with 70 mM of H2O2 for 30 min followed by viability staining. (C) The live/dead ratios of the treated biofilms were calculated using the 3D for LSM (V.1.4.2) software (Carl Zeiss, Inc., Germany).