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. 2018 Oct 22;29(2):137–150. doi: 10.1093/glycob/cwy096

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — N-glycoprofiling of MC38-glycovariants using HILIC-(U)HPLC-FLR-ESI-MS. FLR chromatograms (10–45 min) corresponding to MC38-MOCK (A), MC38-FUT4 (B) and MC38-FUT9 (C) cells. Representative examples of di- or tri-fucosylated glycans bearing the Lewis^x antigen are depicted. The most abundant peaks of the FLR chromatograms represent the high-mannose N-glycans (H5N2/Man5, H6N2/Man6, H7N2/Man7, H8N2/Man8, H9N2/Man9) identified through our analysis. H = hexose (Hex); N = N-acetylhexosamine (HexNAc); F = fucose (Fuc); S = sialic/neuraminic acid (NeuAc).