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. Author manuscript; available in PMC: 2019 Mar 24.
Published in final edited form as: Nat Cell Biol. 2018 Sep 24;20(10):1172–1180. doi: 10.1038/s41556-018-0199-8

Fig. 2. Characterization of in vitro reconstituted microtubule branching.

Fig. 2

(A) Cryo-EM image of branched microtubules. Arrowheads show examples of branching points. Microtubules were stabilized with 1mM GMPCPP. (B) Snapshots of branching microtubules. “Guide-rail” depicts thin lines of density often seen at the split of the branch point. (C) Distribution of branching angles (47±15°, n = 99 branch points). These experiments were performed three independent times.