Table 1.

Lipid Mass, Charge States, and Lipid Stoichiometry Statistics Determined for Native-Like Nanodisc Ions Using FT-Based Approach.

Analyte (Instrument)	Harmonic	Measured Subunit Mass (Da)	z	Lipid Stoichiometry
DPPC-MSP1E3D1 (QTOF)	Fundamental	733. ± 2.	18	273 ± 36†
			19	290 ± 32†
			20	304 ± 28†
			21	316 ± 29†
			22	325 ± 30†
			23	340 ± 41†
			24	353 ± 47
	2nd	733.0 ± 0.8	18	275 ± 41*
			19	294 ± 29
			20	305 ± 26
			21	315 ± 26
			22	327 ± 33
			23	352 ± 46*
			24	383 ± 56*
	Average of fundamental and 2nd harmonic	N/A	18	274 ± 39
			19	292 ± 31
			20	304 ± 27
			21	316 ± 28
			22	326 ± 32
			23	346 ± 45
			24	372 ± 55
DMPC-MSP1D1 (Orbitrap)	Fundamental	678.5 ± 3.6	16	125 ± 15†
			17	129 ± 24†
			18	137 ± 19†
			19	143 ± 22†
			20	149 ± 20†
			21	158 ± 22†
			22	166 ± 23
			23	179 ± 23
	2nd	678.2 ± 1.0	16	128 ± 17
			17	136 ± 14
			18	143 ± 12
			19	146 ± 14
			20	150 ± 16
			21	154 ± 20
			22	162 ± 25
			23	178 ± 28
	3rd	677.3 ± 1.2	16	127 ± 17
			17	137 ± 12
			18	141 ± 12
			19	145 ± 12
			20	150 ± 15

^*indicates Fourier-domain peaks with signal-to-noise less than 10:1

^†indicates Fourier-domain peaks spaced by less than 1.5 times the sum of their apparent standard deviations