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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Chromosoma. 2018 Jul 12;127(4):405–420. doi: 10.1007/s00412-018-0677-6

Fig. 4. Homology-independent targeted integration (HITI).

Fig. 4.

The sgRNA of CRISPR cleaves the same target sequence in the genome and flanking the gene of interest in the donor plasmid (the blue box indicates the beginning of the target sequence and the green box indicates the end of the target sequence). The gene of interest is subsequently inserted into the genomic target site by NHEJ in either orientation. If the sgRNA target site is re-created, the inserted DNA is presumably excised and re-inserted until finally it is in the final stable orientation where the sgRNA target site is no longer present