TABLE 3.
Primary Antibodies
| Antigen | Clone | Dilution | Antigen Retrieval | Source |
|---|---|---|---|---|
| GFAP | GF12-24 | 1:1000 | Microwave/citrate | Chemicon Australia, Boronia, Australia |
| GFAP | Polyclonal | 1:2500 | Microwave/citrate | DakoCytomation Inc., Carpinteria, CA |
| GFAP delta | Polyclonal | 1:1000 | Microwave/citrate | EMD Millipore Corporation, Temecula, CA (Cat. AB9598) |
| AQP4 | Polyclonal | 1:500–1000 | Microwave/citrate | Millipore Corp., Billerica, MA |
| S-100 | Polyclonal | 1:500 | Microwave/citrate | Abcam, Cambridge, UK |
| IgM | Polyclonal | 1:8000 | Proteinase K | DakoCytomation Inc. |
| CD45RO | UCHL1 | 1:150 | Microwave/citrate | Novocastra, Newcastle-Upon-Tyne, UK |
Microwave/citrate, microwave antigen retrieval in a citrate buffer pH6.