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Effects of different MRAPs on hMC4R- or zfMC4R-induced galactosidase basal activity in HEK-293 cells stably expressing galactosidase gene under the control of a constitutive promoter carrying several cAMP-responsive element sites. Data were pooled from all the pharmacological experiments performed to study the effect of melanocortin agonist on hMC4R or zfMC4R (see “Materials and Methods” for details). No significant differences were detected after one-way ANOVA (P < 0.05).
