Table 1.

Effect of MRAP2 on Pharmacological Profile of MC4R

	zfMC4R	zfMC4R + hMRAP2	zfMC4R + zfMRAP2a	zfMC4R + zfMRAP2b
α-MSH	8.25 × 10−9 (4.41 × 10−9–1.50 × 10−8)	9.60 × 10−9 (4.27 × 10−9–2.24 × 10−8)	8.1 × 10−9 (5.32 × 10−9–1.22 × 10−8)	5.58 × 10−9 (2.67 × 10−9–1.07 × 10−8)
hACTH (1-24)	1.27 × 10−7 (5.42 × 10−8–2.92 × 10−7)	5.44 × 10−9 (2.66 × 10−9–1.02 × 10−8)	1.27 × 10−9 (4.66 × 10−10–4.14 × 10−9)	3.81 × 10−8 (2.27 × 10−8–6.28 × 10−8)
	hMC4R	hMC4R + hMRAP2	hMC4R + zfMRAP2a	hMC4R + zfMRAP2b
α-MSH	2.66 × 10−8 (1.78 × 10−8–4.07 × 10−8)	8.63 × 10−9 (5.97 × 10−9–1.23 × 10−8)	7.85 × 10−9 (5.29 × 10−9–1.15 × 10−8)	7.93 × 10−9 (5.93 × 10−9–1.05 × 10−8)
hACTH (1-24)	1.33 × 10−7 (6.89 × 10−8–2.48 × 10−7)	3.52 × 10−10 (1.98 × 10−10–6.34 × 10−10)	4.09 × 10−9 (8.79 × 10−10–1.56 × 10−8)	5.22 × 10−8 (2.91 × 10−8–8.87 × 10−8)

Mean effective dose values (M) of mean reporter activation under the control of cAMP-responsive elements stably expressed in HEK-293 cells transiently expressing hMC4R or zfMC4R alone or in combination with different MRAPs after incubation with melanocortin agonist (hACTH1-24 or α-MSH). The mean of the reporter activation, expressed as percentage of the basal level, for each concentration of melanocortin agonist (hACTH1-24 or α-MSH), was calculated from four replicates of one representative experiment; the resultant data were fitted to logistic curves using GraphPad Prism software. Numbers in parentheses indicate the 95% confidence intervals of nonlinear fittings.