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. 2018 Dec 13;160(2):293–312. doi: 10.1210/en.2018-00797

Figure 6.

Figure 6.

Imaging of ATMs and flow cytometry assessment of ATMs in lean and obese GWAT SVF. (A) Immunofluorescence images depicting CLS labeled for caveolin (Cav; green) and macrophages (Mac2; red) in obese male and female GWAT with and without CL treatment. Scale bar, 500 μm. (B) H&E images of obese male and female GWAT before and after ADRB3 stimulated lipolysis. Scale bars, 500 μm. (C) Graph depicting quantification of CLS numbers per standard unit area and (D) serum IL-6 cytokine levels in mice treated with and without CL. Quantitation of (E) GWAT percentage ATMs, (F) GWAT CD11c+ ATMs, (G) GWAT CD11c− ATMs, and (H) GWAT dendritic cells (DC) numbers. n = 7 to 12 per group. *P < 0.05; **P < 0.01; ****P < 0.0001. Comparisons of M ND PBS vs F ND PBS are shown as ###P < 0.005, and those of M HFD PBS vs F HFD PBS are shown as &&&&P < 0.0001. (I) Immunofluorescence images of male and female obese GWAT depicting Ki67+ proliferating cell nuclei (in red indicated by arrows) and Mac2 labeling of CLS (green) and 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (blue). Scale bar, 500 μm. F, female; M, male.