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| Genes | Initial denaturation | Cycle (32 cycles) | Final extension | ||
|---|---|---|---|---|---|
| One cycle | Denaturation | Annealing | Extension | One cycle | |
| FTO | 95 oC - 5 min | 95o C - 30 sec | 58 oC - 30 sec | 72 oC - 30 sec | 72 oC - 5 min |
| MC4R | 95 oC - 5 min | 95o C- 30 sec | 59 oC - 30 sec | 72 oC - 30 sec | 72 oC - 5 min |
FTO: fat mass and obesity-associated; MC4R: melanocortin-4 receptor.
