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RGC-32 physically interacts with Smad3 in astrocytes. Primary rat astrocytes were treated with vehicle (CTR) or TGF-β (10 ng/ml) for 24 h, and co-immunoprecipitation was performed. Control IgG and anti-Smad3 antibodies were used for immunoprecipitation (IP); anti-RGC-32, -SMAD3 and -SMAD4 antibodies were used for Western blotting (WB). Only Smad3 physically interacted with RGC-32 in astrocytes, and only after stimulation with TGF-β (a). Input is shown in (b)
