Table 1.

Virus-like particles (VLPs) counts under electron microscopy.

Virus-like particles	Particles (per μm2)	Statistics (P < 0.0004)
TG1 VLPs	15.2 ± 8.4	TG1 vs. Multi**
TG4 VLPs	13.3 ± 4.7	TG4 vs. Multi**
TG6 VLPs	8.6 ± 1.9	TG6 vs. Multi**
Combination VLPs (TG1/TG4/TG6 VLPs)	12.4 ± 6.2	Comb vs. Multi**
Multi-antigen VLPs (TG146 VLPs)	22.9 ± 9.4

Virus-like particles were counted under transmission electron microscopy (TEM). Combination VLPs (TG1/TG4/TG6) expressing three proteins (TG1 for IMC, TG4 for ROP, and TG6) were generated with each VLP expressing IMC, MIC8, or ROP18 together with influenza M1 as core protein as described in materials section. Multi-antigen VLPs (TG146 VLPs) were generated by co-infecting Sf9 cells with rBVs expressing TG1, TG4, and TG6 proteins together with influenza M1 as core protein as described in materials section. Significantly higher number of particles was found in multi-antigen VLPs compared to combination VLPs or single VLPs (TG146 VLPs vs. TG1/TG4/TG6 VLPs, TG1 VLPs, TG4 VLPs, TG6 VLPs,

^**P < 0.0004).