FIGURE 3.

Histological analysis of posterior segments of the dark-adapted and illuminated in vivo rat eyes. Representative paraffin-embedded sections of the eye tissues were stained with hematoxylin and eosin (magnification 400×, scale bar 50 μm). Designations are as follows: GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear retinal layer; Ph, photoreceptor layer. (A) The retina of a control dark-adapted animal. (B) The retina isolated immediately after illumination for 14 h with metal halide lamp (2,500 lx). ONL migration in the vitreal direction is indicated by black arrow. (C,D) The retina isolated 7 days after the light exposure. ONL disorganization and thinning are indicated with white arrows. INL migration and thinning of OPL and INL are marked on (C,D) with gray and black arrows, respectively.