FIGURE 3.

Puerarin preferentially blocks resting Na_v channels in DRG neurons of paclitaxel (day 10) rats (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) The currents were recorded before and 5, 10, 15 min after the external application of vehicle or puerarin at corresponding IC₅₀ concentrations. Currents traces evoked from a holding potential of –90 mV to the test pulse of –10 mV (insert). (B) The summary data show the inhibitory rates of puerarin in indicated groups and time points after puerarin application (C, control; P, puerarin; V, vehicle; n = 8 neurons in each group). ^∗P < 0.05, ^∗∗∗P < 0.001 compared with corresponding C+V groups; ^##P < 0.01, ^###P < 0.001 compared with corresponding 0 min. (C) IC₅₀ values of puerarin on resting Na_v channels of DRG neurons in control (n = 8 neurons in each data point) and paclitaxel (n = 8 neurons in each data point) groups. (D,E) The currents were recorded before and 15 min after puerarin application at corresponding IC₅₀ concentrations in control (D) and paclitaxel (E) groups. n = 9 neurons in each group.