FIGURE 5.

Puerarin use-dependently blocks Na_v channels of DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A–F) The amplitudes of I_Na,peak evoked by 100 pulses (from a holding potential of –90 mV to the test pulse of –10 mV, 40 ms) were normalized to those of the first response and plotted against pulse number. The normalized amplitudes at different frequencies in indicated groups were recorded before and 15 min after puerarin application at corresponding IC₅₀ concentrations. (G) The comparison of the net inhibitory rates calculated with responses elicited by 100th pulses at indicated frequencies in control and paclitaxel (day 10) groups are shown. n = 9 neurons in each test. ^∗∗P < 0.01, ^∗∗∗P < 0.001.