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. 2019 Jan 7;12:115. doi: 10.3389/fnana.2018.00115

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Copyright © 2019 Padmanabhan, Osakada, Tarabrina, Kizer, Callaway, Gage and Sejnowski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modified g-deleted rabies tracing to identify feedback projection to the olfactory bulb. (A) Schematic outlining experimental design. (B1) Neurons labeled with mCherry rabies (red) and DAPI nuclear counter-stain (blue) at the injection site. Scale bar = 200 um. mi, mitral cell layer; opl, outer plexiform layer; ipl, inner plexiform layer; gl, glomerular layer; gcl, granule cell layer. (B2) Enlargement of neurons in (B1) shows clearly labeled neurons in the GCL. (C) Outline of approach involves automated fluorescence microscopy, coronal section alignment, automated cell counting, and subsequent analysis. (D) Example of coronal sections used for whole brain reconstructions.

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