Figure 6.

Comparison of Differential Regulation between Th17 and Th0 at 72 hr in Human and Mouse

(A–D) Venn diagrams demonstrating (A) the total number of common and unique detections in human and mouse proteomics data and (B) the number of common and unique differentially regulated proteins detected. The numbers of differentially regulated proteins behaving in the similar or opposite fashion is shown in the overlap. Differentially regulated proteins refer to those proteins detected as DE or only in one condition. The Z score standardized expression levels of the commonly detected differentially regulated proteins regulated (C) similarly or (D) in an opposite fashion in human and mouse.

(E) Immunoblot validations of SATB1 and CD44 expression in human and mouse during Th17 cell polarization. Blots show the protein extracts from the Th0 and Th17 cells at 72 hr. A representative blot from three biological replicates is shown.

(F) The logarithmic fold changes (logFC) of all the common detections in human and mouse. The DE proteins detected as DE also in mouse are marked with black, and those uniquely DE in humans are marked with red. The Pearson correlation coefficient for the logFCs of all the common detections was 0.102 (p value < 0.001, n = 3,143), 0.304 (p value = 0.46, n = 8) with the common DE proteins detected, and 0.34 (p = 0.04, n = 73) for the DE findings unique to humans among the common detections.

(G) The logFCs and identities of the 25 most up- and downregulated DE proteins in humans and mouse. The common detection is marked with **. Proteins detected as DE in the other species but not among the 25 most up- and downregulated are marked with *. Mouse genes corresponding to mouse proteins are transformed as orthologous human genes for comparison using Ensembl BioMart. Black color in the heatmaps stands for undetected/missing value.