Skip to main content
. 2018 Dec 26;11:334–355. doi: 10.1016/j.isci.2018.12.020

Figure 7.

Figure 7

SATB1 Is a Negative Regulator of Human Th17 Cell Differentiation

The mRNA expression profile of SATB1 in Th0 and Th17 cells at 48 and 72 hr after cell activation (A). Western blot analysis of SATB1 expression in Th0 and Th17 cells at 48 and 72hr after cell activation (B). The RNA interference (RNAi)-mediated knockdown of SATB1 at 24 hr post-Th17 cell polarization using specific siRNAs. Scramble siRNA (SCR) was used as a negative control. Silencing of SATB1 at mRNA (C) and protein (D) level was measured. Effect of SATB1 knockdown on IL-17A and IL-17F mRNA levels (E) by real-time TaqMan PCR analysis and on IL-17A protein secretion (F) was quantitated by ELISA assay from SATB1 siRNA nucleofected Th17 cells relative to NT-siRNA at 72 hr post-initiation of Th17 polarization. CCR6 surface expression detected by flow cytometry was measured (G) from SATB1 siRNA nucleofected Th17 cells relative to NT-siRNA at 72 hr post-initiation of Th17 polarization. Data are representative of 3–5 independent experiments. Error bars represent standard deviation values across biological replicates (n = 3–5; *p < 0.05, **p < 0.01 for Th0 versus Th17 and SCR versus siSATB1; two-tailed t-test analysis).