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. 2019 Jan 11;10:17. doi: 10.1186/s13287-018-1111-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Construction of Fstl1-overexpressing MSCs. a Transduction efficiency of engineered MSCs evidenced by mCherry reporter expression. Scale bar = 50 μm. b Fstl1 transcription in engineered MSCs was assessed by qRT-PCR (n = 4). c Protein level of Fstl1 in engineered MSCs was determined by western blot analysis. d Flow cytometry analysis of cell surface antigens of genetically modified MSCs. Unstained and isotype controls are indicated by red and green spectra, respectively. **P < 0.01; ***P < 0.001