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. 2019 Jan 11;10:17. doi: 10.1186/s13287-018-1111-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Fstl1 facilitates survival and proliferation of MSCs under hypoxic condition. Engineered MSCs were challenged with hypoxia for 48 h. a Flow cytometry of Annexin V-PE/7-AAD staining. Etoposide-treated cells were used as positive controls. b Cell proliferation detected using EdU incorporation assay. Unstained control is indicated by red spectra. c Western blot analysis of p-Akt (Ser473), Akt, p-GSK-3β (Ser9), and GSK-3β in hypoxic MSCs