Fig. 4.

Competition of SEPW1P RNA with SEPW1 mRNA for miR-7 and miR-324. a Relative luciferase activity of psiCHECK2 vector bearing SEPW1 3’UTR in MCF7 and ZR75–1 cells with overexpression or knockdown of SEPW1P by cotransfected with pcDNA3.1-SEPW1P or siSEPW1P. b Relative reporter gene activity of psiCHECK2 vector bearing SEPW1P in MCF7 and ZR75–1 cells with overexpression or knockdown of SEPW1 by cotransfected with pcDNA3.1-SEPW1 or -siSEPW1. c Levels of SEPW1 mRNA and protein in MCF7 and ZR75–1 cells with overexpression (pcDNA3.1-SEPW1P) or knockdown (siSEPW1P) of SEPW1. d Relative reporter gene activity of constructs bearing SEPW1P (d) or SEPW1 3’UTR (e) in MCF7 and ZR75–1 cells cotransfected with miR-7 or miR-324 mimic or inhibitor. f, g Relative reporter gene activity of psiCHECK2 vector bearing SEPW1P (f) or SEPW1 3’UTR (g) with mutations at putative binding site of miR-7 or miR-324 in MCF7 and ZR75–1 cells cotransfected with miR-7 or miR-324 mimic or inhibitor. Shown are mean ± SEM; **, P < 0.001; ***, P < 0.0001