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. 2018 Jun 19;17(1):75–87. doi: 10.1111/pbi.12948

Figure 5.

Figure 5

Schematic representation of proposed model for extraction procedure with a biorefinery approach of triterpenes from Phaeodactylum tricornutum and Saccharomyces cerevisiae biomass. The extraction procedure for triterpenoids from P. tricornutum simultaneously isolates more commercially relevant coproducts compared to the yeast S. cerevisiae. Cost of extraction is estimated to be assuming equal levels of extraction efficiency. Cost of biomass for P. tricornutum is higher (produced in Europe, Necton), but market sizes of lipids omega 3 and carotenoids (unique products in microalgae) create cost offsets. References for panel A: a: *W. Yongmanitchai and O. P. Ward Growth of and Omega‐3 Fatty Acid Production by Phaeodactylum tricornutum under Different Culture Conditions Appl. Env. Micro., Feb. 1991, p. 419–425 0099‐2240/91/020419‐07$02.00/0, b: Yu‐Hong Yang, Lei Du, Masashi Hosokawa, Kazuo Miyashita, Yume Kokubun, Hisayoshi Arai and Hiroyuki Taroda. Fatty Acid and Lipid Class Composition of the Microalga Phaeodactylum tricornutum J. Oleo Sci. 66, (4) 363–368 (2017) https://doi.org/10.5650/jos.ess16205, c: this paper, d: S. M. Tibbetts, J. E. Milley, P. Santosh and J. Lall Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors J Appl Phycol (2015) 27:1109–1119. References for panel B: e: International Journal of Environment, Agriculture and Biotechnology (IJEAB) Vol‐2, Issue‐2, Mar–Apr 2017 https://doi.org/10.22161/ijeab/2.2.2 ISSN: 2456–1878 Page | 558, f: M. Lamac Ka and J. S Ajbidor Ergosterol determination in Saccharomyces cerevisiae. Comparison of different methods. Biotech. Tech. 11, 723–725 (1997), g: E. Czarnotta, M. Dianat, M. Korf, F. Granica, J. Merz, J. Maury, S. A. Baallal Jacobsen, J. Förster, B. E. Ebert, and L. M. Blank. Fermentation and purification strategies for the production of betulinic acid and its lupane‐ type precursors in Saccharomyces cerevisiae. Biotech. Bioeng. 114, 2528–2538 (2017) https://doi.org/10.1002/bit.26377