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. 2018 Jun 19;17(1):75–87. doi: 10.1111/pbi.12948

Figure 7.

Figure 7

Betulin production in Phaeodactylum tricornutum transformant lines. (a) GC‐MS analysis of WT (blue), LjLUS‐25 containing only the Lotus japonicus lupeol synthase (LjLUS) (red); transformant line with constructs harbouring L. japonicus lupeol synthase (LjLUS), Medicago truncatula cytochrome P450 (MtCYP716A12) and cytochrome P450 reductase (MtCPR) in WT background (purple); transformant line with construct harbouring fused P450‐reductase protein MtCYP716A12λΔ72CPR in LjLUS‐25 background (light blue); standards (black). Peak as indicated 1. Brassicasterol; 2. Lupeol; 3. Betulin. (b) Detail of CYP716A enzyme reaction. The oxidation at C28 leads to betulin intermediate, which is converted to betulin aldehyde, and betulinic acid.