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. 2018 Jun 25;17(1):206–219. doi: 10.1111/pbi.12958

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© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Comparison of Populus trichocarpa v3.0 (Reference) and PacBio Iso‐Seq data isoform annotations. (a) Identified isoforms in three samples (Apex, IN1‐3 and IN4‐5). (b) Distribution of the percentage transcripts with different exon numbers for reference and PacBio Iso‐Seq data. (c) Comparison of gene model and PacBio Iso‐seq isoform length. (d) A pie chart showing the percentage of PacBio Iso‐Seq isoforms that are the same as existing gene models, novel isoforms of known genes and novel isoforms of novel genes.