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. 2018 May 31;17(1):63–74. doi: 10.1111/pbi.12947

Figure 4.

Figure 4

GhL1L1 binds to the G‐box in the promoter to activate the expression of GhPP2AA2. (a) Schematic diagram of different regions of the promoter of GhPP2AA2, named A, B, C. (b) proPP2AA2‐F represents the full length of the promoter, proGhPP2AA2‐ΔC represents deletion of the A region, proGhPP2AA2‐ΔG represents deletion of the B region and proGhPP2AA2‐mG represents mutation of the B region (G‐box, CACGTT mutated to CAAGGT). (c) Transformant yeast colonies visible on the medium (SD‐Trp‐His + 3‐AT) show that GhL1L1 was able to bind to proPP2AA2‐F and proGhPP2AA2‐ΔC. No visible yeasts were observed when co‐transforming GhL1L1 either with proGhPP2AA2‐ΔG or proGhPP2AA2‐mG . Empty pDEST22 vector was used as a negative control. 3AT, 3‐Amino‐1,2,4‐triazole. (d) Schematic diagram of effectors and reporter. GhL1L1 activates gene expression by binding to the G‐box element in the promoter of GhPP2AA2 in tobacco leaf protoplasts (e) and OE4 and null EC protoplasts (f) in vivo. 62‐SK (‐GhL1L1) served as negative controls. Data are shown as the mean ± SE (n = 3). Different capital letters denote significant differences by multiple comparisons using Statistix 8.0 software.