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. 2018 May 31;17(1):63–74. doi: 10.1111/pbi.12947

Figure 6.

Figure 6

TIBA treatment affects callus proliferation and auxin distribution during cotton SE. (a) The phenotypes of different GhL1L1 transgenic and null lines at 15 and 25 days postinduction treated with TIBA, scale bars = 5 mm. (b) The CPR of different transgenic and null lines at 15 and 25 days postinduction treated with TIBA. Different capital letters denote significant differences by multiple comparisons using Statistix 8.0 software. (c) ECs or embryos were observed from the transgenic and null lines (white arrows) at 50 days postinduction treated with TIBA, scale bars = 2 mm. (d) The embryonic differentiation rate (EDR) of different transgenic and null lines at 40, 50, 60 and 80 days postinduction treated by TIBA. qRT–PCR analysis of GhABI3 (e, f) and GhFUS3 (g, h) in GhL1L1 transgenic and null explants induced on MSB medium or supplemented with TIBA at 25, 40 days postinduction. The data (in b, d, e, f, g and h) are shown as the mean ± SE (n = 3).