Figure 3.

GmmiR156b overexpression shortens the length of the plastochron to increase the number of trifoliolate leaves and nodes per plant. (a) Transgenic miR156b OE‐5, miR156b OE‐11 and wild‐type control plants were grown in a field at 70 days after emergence (DAE). Scale bar, 20 cm. (b) The total trifoliolate leaf number per transgenic and wild‐type plant (n = 20). (c) Dynamic changes in the trifoliolate leaf number on the main stem in wild type and miR156b OE transgenic lines (n = 10). (d) The leaf initiation rates were significantly increased in the miR156b OE lines (n = 10). (e) Average node number per transgenic and wild‐type plant (n = 20). All data in the graphs represent means ± SEs. Statistical significance was estimated by Student's t‐tests. *P < 0.05; ***P < 0.001.