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. 2018 May 23;17(1):50–62. doi: 10.1111/pbi.12946

Figure 5.

Figure 5

5′ RACE validation of the target genes of GmmiR156b and expression analyses. (a) Experimental validation of miR156b target genes and cleavage sites using 5′ RACE. Vertical arrows indicate the 5′ termini of the miRNA‐guided cleavage products, as identified by 5′ RACE, with the frequency of clones shown. (b and c) qRT‐PCR analysis of GmSPL expression in the shoot apex (a) and axillary buds (b) of miR156b OE‐5 and wild‐type plants. GmELF1b was used as an endogenous control for gene expression. Asterisks represent significantly decreased expression of the GmSPL genes in miR156 OE‐5 plants (Student's t‐test; **P < 0.01; ***P < 0.001).