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. 2018 Oct 11;195(2):226–236. doi: 10.1111/cei.13218

Figure 3.

Figure 3

Signaling pathways involved in Yin Yang 1 (YY1)‐induced interleukin (IL)‐8 production in lymphocytes of rheumatoid arthritis (RA). (a) Peripheral blood mononuclear cells (PBMC) isolated from RA patients were treated with lentivirus‐control (LV‐NC), LV‐YY1‐short hairpin (sh)RNA or LV‐YY1. Then, 50 μM LY294002 (inhibitor of PI3K), 100 μM BAY 11‐7082 [inhibitor of nuclear factor kappa B (NF‐κB)], 50 μM SB203580 [inhibitor of mitogen‐activated protein kinase (MAPK)], 10 μM SP600125 [inhibitor of Janus kinase (JNK)] and 50 μM rapamycin [inhibitor of mammalian target of rapamycin (mTOR)] was added to LV‐YY1‐treated PBMC. After 48 h, cell supernatant was collected for detection of IL‐8 by enzyme‐linked immunosorbent assay (ELISA). (b) Western blot analysis of the PI3K, Akt and phosphorylated PI3K, Akt expression in PBMC treated with LV‐NC or LV‐YY1‐shRNA. The grey value of p‐Akt/Akt and phosphatidylinositol‐3‐kinase (p‐PI3K)/PI3K was calculated by Image J.