Skip to main content
. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Infect Genet Evol. 2018 Jun 19;64:95–104. doi: 10.1016/j.meegid.2018.06.019

Fig. 1.

Fig. 1.

Study designs for FER-16–09 (A) and FER-17–01 (B). In FER-16–09 experiment, 7 groups of 3 ferrets were vaccinated with 105 EID50 in 500 uL with LAIVs (HK 6:2, HK 5:3, Sw SW 6:2, Sw SW 5:3, Tx TX 6:2, Tx TX 5:3) or PBS (mock) intranasally under anesthesia on day 0. On day 21 post-infection (DPI), ferrets were intranasally challenged with 103 TCID50 in 500uL of A/Hong Kong/4801/2014 (H3N2) virus. In the FER-17–01 experiment, 3 groups of 8 ferrets were vaccinated with 107 EID50 in 500 uL of LAIV (Tx 6:2 or Tx 5:3) or PBS (mock) intranasally under anesthesia on day 0. At 30 DPI, ferrets were intranasally challenged with 107 TCID50 in 1000uL of A/Brisbane/10/2007 (H3N2) virus. Animals were bled prior to LAIV vaccination, prior to wild-type virus challenge and at the end of the experiment. Nasal fluid washes were collected every second day after LAIV or wild-type virus infection as indicated. Nasal, laryngeal, tracheal, caudal left lung lobe and caudal right lung lobe tissue samples were collected from the four animals in each group on days 32 and 35.