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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Mol Pharm. 2018 Oct 17;15(11):5336–5348. doi: 10.1021/acs.molpharmaceut.8b00802

Figure 5. Confocal microscopic analysis of time-dependent Texas Red/Ace-DEX MPs co-localization to the lysosomes and cytosolic escape.

Figure 5.

(A) Ace-DEX MPs composed of Texas Red-conjugated Ace-DEX were delivered to bone marrow-derived macrophages at set time points, followed by live cell imaging using laser scanning confocal microscopy (40× oil immersion). Red: Texas Red microparticles, green: LysoTracker Green dye. Representative image of three independent experiments. Scale bar is 10 μm. B) Co-localization was quantitatively measured by computing co-localization coefficients (Manders Overlap) using Image J and the plug-in JaCop. Values are reported as mean ± SEM.