Figure 1.

Efficiency of interference and overexpression of BMAL1 in pinealocytes under green light. (A) Fluorescent dye-labelled siRNA (100 pmol/well; FAM fluorescent) was transfected into chick pinealocytes to evaluate the transfection efficiency. (B and C) The expression of BMAL1 mRNA and cBMAL1 protein after transfecting si-BMAL1 and the negative control siRNA. (D and E) The expression of BMAL1 mRNA and cBMAL1 protein after transfecting pcDNA3.1-BMAL1 and pcDNA3.1. The results are presented as the mean ± s.e.m. N = 3 per time point. Significant differences between different treatment groups are shown with asterisks, where *P < 0.05, **P < 0.01 and ***P < 0.001. (A–C) Scale bar = 50 μm.