Figure 3.

Temporal changes in AANAT mRNA and melatonin secretion after BMAL1 and CLOCK interference and overexpression in pinealocytes under green light. Pink points and lines represent the BMAL1 interference group, blue points and lines represent the CLOCK interference group, yellow points and lines represent the BMAL1 overexpression group and purple points and lines represent the CLOCK overexpression group. The horizontal white bar on each figure represents the day, and the horizontal black bar represents the night. The solid points indicate the relative mRNA levels or the plasma melatonin levels. The curved lines indicate the rhythmic levels of genes and melatonin by the cosinor analysis. The horizontal dotted line represents the mesor. The vertical solid line represents acrophase and amplitude (the distance between maximum and mesor). The results are presented as the mean ± s.e.m. N = 3 per time point.