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. 2019 Jan 7;9:734. doi: 10.3389/fpsyt.2018.00734

Figure 3.

Figure 3

The regulation of fluoxetine on the level of BDNF in primary culture astrocytes. In the primary cultured astrocytes, after the pretreatment with or without 200 nM SB204741 (SB), 10 μM U0126 or 10 μM LY294002 (A,C), or after RNA interfering the expression of 5-HT2B receptors or cfos with siRNA duplex (B,D), then the astrocytes were treated with PBS or fluoxetine for 1 week. Representative blots for the expression of BDNF was shown in (A,B). Average BDNF level was quantified as the ratio between the BDNF and β-actin, n = 6 (the upper panels of C and D). Meanwhile, the average BDNF level was also measured via ELISA, n = 6 (the lower panels of C and D). *p < 0.05, statistically significant difference compared with any other group except for each other (B); *p < 0.05, statistically significant difference compared with any other group (D).