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. 2019 Jan 7;9:734. doi: 10.3389/fpsyt.2018.00734

Figure 5.

Figure 5

The regulation of leptin on the effects of fluoxetine in astrocytes. After RNA interfering the expression of leptin receptors or cfos with siRNA duplex, the primary cultured astrocytes were pre-treated with PBS (Control) or leptin, then the cells were treated with PBS or fluoxetine for 30 min to measure the phosphorylation of ERK1/2 or for 1 week to check the expression of the other proteins (A–C). Representative blots for the level of p-ERK1/2, cfos and BDNF were shown in (A). The average phosphorylation of ERK1 and ERK2 was quantified as the ratio between the p-ERK1 and ERK1 or p-ERK2 and ERK2, n = 6 (the left and middle panels of B), the average expression of cfos and BDNF were normalized by β-actin, n = 6 (the right panel of B and the left panel of C). The average BDNF level measured via ELISA was shown in (B), n = 6. *p < 0.05, statistically significant difference compared with any other group except for each other (B,C); **p < 0.05, statistically significant difference compared with any other group (B,C).