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. 2019 Jan 7;12:516. doi: 10.3389/fncel.2018.00516

FIGURE 4.

FIGURE 4

Overexpression of IL-1β within the vmPFC leads to depression-like phenotypes in normal wild type rats. (A) Left: Schematics of AAV vectors engineered to overexpress a control construct or IL-1β. ITR, inverted terminal repeats; EF1, ZsGreen promoter; CMV, IL-1β promoter. Right: Experimental paradigm for behavioral biochemical testing of rats infected with the virus. (B) Illustration of viral infusion of AAV-IL-1β into the vmPFC. PrL, prelimbic cortex; IL, infralimbic cortex. Scale bar is 100 μm. (C) Estimation of βCaMKII overexpression efficiency after viral constructs injection. (D,E) Behavioral effects of over expressing viral AAV-IL-1β in the vmPFC. N = 12 in all groups. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 WT + AAV-IL-1β vs. WT rats. #P < 0.05, ##P < 0.01, ###P < 0.001 WT + AAV-IL-1β + Cur vs. WT + AAV-IL-1β (WT, wild type).