Fig. 2.

The effects of CXCL2 overexpression on the cell cycle. (A) Representative FACS images of HCCLM3 and MHCC97H cells with LV-CXCL2 or LV-GFP. (B) Alterations in cell cycle distribution were analysed. **P < 0.01 and *P < 0.05. (C) Western blot analysis revealed the effect of CXCL2 on the expression of cyclin-dependent kinase and cyclin proteins. (D) Gene expression microarray showed 6908 differentially expressed genes. (E) The KEGG analysis indicated that CXCL2 regulated metabolic, PI3K—Akt signalling, cell cycle, pathways and so on. (F) CXCL2 reduced ERK1/2 phosphorylation in HCC cells.