Fig. 3.

CXCL2 induces apoptosis in HCC cell lines and inhibits the growth of tumours in vivo. (A) Apoptosis rates of HCC cells transfected with CXCL2 compared with control as assessed by flow cytometry. (B) Quantitative analysis of apoptosis cells (Q2 + Q4, *P < 0.05). (C) Protein expression of pro-apoptosis mediators and anti-apoptosis regulators in HCC cells were determined by Western blot. (D) Photographic images of the subcutaneously formed tumour tissues. (E) LV-CXCL2-transfected HCCLM3 exhibited significantly reduced tumour volume and weight. *P < 0.05. (F) Representative images of CXCL2 protein expression as assessed by immunohistochemistry.