Differential expression of Caspase-3 was observed in the GBM patients with some of this expressing greater levels respect to basal expression in control tissue (A), while Caspase-9 levels were consistently lower in human tumor than in control tissue (B). Moreover, an increased expression levels of p-JNK was observed in tumor samples compared to healthy samples (C). The TLR-4 expression was significantly increased in GBM patients tissue (D) respect to control brain tissue (D). Also, FasL expression was notably higher in GBM samples (E). Each data are expressed as mean ± SEM of patients. T test, Unpaired T test. *p < 0.05, **p < 0.01 and ***p < 0.001 vs Ctr. Caspase 3, caspase 9 and fas-ligand were detected in the same stripped membrane (A, B and E) Therefore, the ubiquitary GAPDH band is the same for all three antibodies.