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A diffuse expression of both TLR-4 and Dkk-3 in U-138MG (A and C) and in A-172 (B and D) cell lines was observed by immunofluorescence analysis. Cell nuclei were stained in blue with DAPI (E and F). Yellow spots are indicative of a significant TLR-4/Dkk-3 co-localization (G and H).
