Fig. 2.
Representative non-denaturing SDS-PAGE images demonstrating the outcome of AF-maleimide labeling of tau K18 in reducing and non-reducing conditions. Migration of tau K18 labeled with AF-maleimide with (A) or without (B) prior TCEP treatment. The figures show representative (n = 3) non-denaturing SDS-PAGE analysis of tau aggregate patterns in the two labeling methods.