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. 2019 Jan 10;11:637–649. doi: 10.2147/CMAR.S185117

Figure 6.

Figure 6

HMGA1 was a direct target of miR-4458.

Notes: (A) The predicted binding site of miR-4458 in the 3′-UTR of HMGA1 from miRNA website. (B) The analysis of luciferase activity after different combinations of miR-4458, miR-NC, h-HMGA1-WT, and h-HMGA1-MUT. (C) The expression of HMGA1 in NSCLC cells after 48 hours of transfection with miR-4458 NC, mimics, inhibitor NC, and inhibitor. (D) Western blot analysis of HMGA1 in NSCLC cells after 48 hours of transfection with miR-4458 NC, mimics, inhibitor NC, and inhibitor. *P<0.05, **P<0.01.

Abbreviations: miR-4458, microRNA-4458; NC, negative control; NSCLC, non-small-cell lung cancer.