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. 2019 Jan 10;11:611–624. doi: 10.2147/CMAR.S182719

Figure 5.

Figure 5

SNHG6 binds to miR-181a-5p.

Notes: (A) The predicted binding site of miR-181a-5p in SNHG6, and mutation of the binding sequence in SNHG6. (B) Luciferase activity assays in 293T cells co-transfected with miR-181a-5p mimics and the indicated SNHG6-WT or SNHG6-MUT. *P<0.05. (C) The expression of miR-181a-5p was examined by qRT-PCR in HCT116 and SW480 cells transfected with sh-NC or shRNA1 and shRNA2 targeting SNHG6. *P<0.05 and **P<0.01. (D) The expression of miR-181a-5p was examined by qRT-PCR in SHNG6 sh-NC, shRNA1, and shRNA2 xenograft tumors. *P<0.05 and **P<0.01. (E) The expression of SNHG6 was examined by qRT-PCR in HCT116 and SW480 cells transfected with miR-control or miR-181a-5p mimics. *P<0.05. (F) The expression of SNHG6 was examined by qRT-PCR in HCT116 and SW480 cells transfected with miR-NC or miR-181a-5p inhibitors. *P<0.05. (G) Expression levels of miR-181a-5p in a normal colorectal epithelial cell line (NCM460) and CRC cell lines (HT-29, HCT116, SW480, SW620, and CACO2). (H) The correlation between SNHG6 and miR-181a-5p in 141 CRC tissues. (I and J) Cell proliferation was measured by MTT assays in HCT116 and SW480 cells transfected as indicated. *P<0.05.

Abbreviations: CRC, colorectal cancer; qRT-PCR, quantitative real-time PCR.