Fig 2. Rab32, Rab38 and SNX6 binding in co-immunoprecipitation and GST-pulldown experiments.

(A) Lysates from IHKE-1 cells stably expressing GFP-Rab32 were used for co-immunoprecipitation of endogenous SNX6 with the GFP-Trap kit. IHKE-1 cells transiently transfected with GFP alone were used as control. n = 2 independent experiments. (B) 5 μg GST-Rab32 wt, GST-Rab38 wt, GST-Rab1A wt or GST as control were loaded to glutathione agarose beads followed by incubation with IHKE-1 lysate overnight. Samples were analyzed by SDS-PAGE and subsequent Western blot analysis against SNX6. n = 3 independent experiments.