Table 2. Structures of N-2/3 Derivatives 8, 10, and 13 and Their Effects on the KCNQ2, 4, and 5 Channels.

			I/I0a
Cpds	R2	R3	KCNQ2	KCNQ4	KCNQ5
8a	Me	Me	1.37 ± 0.11	4.77 ± 0.44	2.89 ± 0.07
8b	allyl	allyl	0.67 ± 0.02	2.21 ± 0.11	5.26 ± 0.53
8c	nPr	nPr	0.78 ± 0.04	2.58 ± 0.56	2.75 ± 0.84
8d	iPr	iPr	0.35 ± 0.04	1.33 ± 0.06	3.11 ± 0.93
8e	nBu	nBu	0.77 ± 0.02	2.75 ± 0.40	4.13 ± 1.09
8f	iBu	iBu	0.83 ± 0.04	1.52 ± 0.17	1.73 ± 0.10
8g	tBu	tBu	1.00 ± 0.09	3.05 ± 0.74	5.32 ± 0.39
8h	cyclopropyl	cyclopropyl	0.64 ± 0.03	1.51 ± 0.04	2.55 ± 0.38
8i	cyclobutyl	cyclobutyl	0.26 ± 0.06	1.21 ± 0.09	1.33 ± 0.23
8j	cyclopentyl	cyclopentyl	0.21 ± 0.05	0.88 ± 0.07	1.25 ± 0.14
8k	Ph	Ph	0.91 ± 0.02	1.22 ± 0.05	1.16 ± 0.04
8l	Bn	Bn	1.25 ± 0.01	3.02 ± 0.28	3.57 ± 0.31
10a	Et	Me	0.61 ± 0.05	2.56 ± 0.47	3.34 ± 0.54
10b	Et	Allyl	0.96 ± 0.04	3.68 ± 0.36	4.67 ± 0.69
10c	Et	nPr	1.07 ± 0.03	3.95 ± 0.89	5.81 ± 0.62
10d	Et	iPr	0.94 ± 0.06	5.01 ± 0.83	5.71 ± 0.34
10e	Et	nBu	1.06 ± 0.03	4.16 ± 0.60	3.62 ± 0.31
10f	Et	iBu	1.04 ± 0.04	5.19 ± 0.64	4.84 ± 0.32
10g	Et	tBu	1.16 ± 0.04	6.37 ± 0.84	4.58 ± 0.32
10h	Et	Bn	0.97 ± 0.15	1.27 ± 0.03	2.57 ± 0.67
13	tBu	Et	1.07 ± 0.06	7.71 ± 0.50	4.59 ± 0.56

^aThe testing concentration was 10 μM. Each compound was tested in more than four cells.